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KMID : 1123920030170020525
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 2 p.525 ~ p.528
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Inhibitory effect of EtOAc Subfraction-2 of Spatholobus Suberectus Dunn. on tumor Metastasis
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Lee Hyun-Chul
Huh Jeong-Eun
Lee Jong-Soo
Ryu Shi-Yong
Kim Sung-Hoon
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Abstract
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Spatholobus Suberectus dunn. has been applied to blood stasis in oriental medicine. We selected one potent ethyl acetate subfraction-2 from Spatholobus Suberectus Dunn.(SSD) from anti-metastasis screening. It exerted the cytotoxicity against HT1080 and B16BL6 with the IC50 of 60 ug/ml and also significantly inhibited tumor cell induced platelet aggregation (TCIPA). It effectively didn’t inhibit cell adhesion of HT1080 to matrigel coated wells, while it inhibited the cell invasion of HT1080 at the doses of 10, 20, 40 μg/ml by Boyden chamber assay. It effectively suppressed lung metastasis by B16BL6 melanoma in C57BL6 mice. These results indicate that the EtOAc subfraction-2 of Spatholobus Suberectus Dunn. can be applied to caner treatment with anti-metastatic activity.
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KEYWORD
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Metastasis, Cytotoxicity, TCIPA, Invasion
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